Composite

Part:BBa_K2224002

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


Promoter+RBS+Lecanicillium lecanii strain CA-12 cuticle-degrading proteinase (Pr1)+Terminator

This composite part contains a coding sequence of proteinase , which is from the Lecanicillium lecanii strain CA-12. The Lecanicillium lecanii uses this proteinase to degrade the protein-chitin shell of scale insect. This part can be transformed into E.coli to express proteinase, which shows a strong ability in degrading the protein layer under the Scanning Electron Microscope. In SMS_Shenzhen 2017 project, this proteinase functions as a core mechanism to degrade the chitin-protein complex,in which the protein wraps up the chitin to form the hard layer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 101
    Illegal AgeI site found at 462
    Illegal AgeI site found at 1128
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None